Abstract
The pattern of differentiation of the enterocyte along the villus is considered to be dependent on the age of the cell and uniform in cells occupying comparable location on the villus. Using a polyclonal antibody against A blood group specific components of enterocyte brush border and three monoclonal antibodies we have demonstrated the expression of the A and B antigens in the brush border and Golgi apparatus of enterocytes of individuals of the expected blood groups, by light and electron microscopy, with the immunogold technique. The polyclonal anti-A serum was specific for the brush border whereas the monoclonal reagents also bound the endothelial cells and erytrocytes in the sections. Four of 16 blood group A individuals showed a mosaic pattern of expression of the A antigen, both with the polyclonal serum and the monoclonal anti-A reagent. Some enterocytes showed a normal staining and some other enterocytes at similar position on the villus showed no staining at all. An anti-Le a antibody showed that all the 4 individuals with the mosaic pattern were non secretor. Abnormalities of blood group antigens may reflect abnormal expression of glucosyl-transferase genes or disturbances of their acceptors. Enterocytes may therefore display a non homogeneous pattern of differentiation in morphologically similar cells occupying comparable location on the villus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
