Abstract
Granulosa cells, obtained from preovulatory follicles of PMS-LH treated immature rats, were cultured as monolayers in 90% medium 199, 10% human male serum and antibiotic. Purified rFSH, rGH, rPRL, hLH or hPL was added separately to the culture medium. Control cultures lacked the above hormones. Progesterone secreted into the culture medium was measured by specific radioimmunoassay and served as an index of luteotrophic stimulation. During a 2 week culture period, rPRL and hPL, but not rFSH, rGH or hLH, brought about complete luteinization as observed by electron microscopy and enhanced progesterone secretion over that of control cultures. The transformation of lamelliform to villiform cristae within mitochondria was the most striking ultrastructural feature indicative of luteotrophic stimulation and progesterone secretion. Rat granulosa cells were also cultured with 0.01, 0.1 and 1.0 μg/ml of rPRL alone or in combination with 0.5 fig rFSH, 0.1 μg hLH or 4.0 ng estradiol (E2)/ml. Cultures maintained with t...
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