Abstract
In this work, specific concentrations of cadmium, copper and zinc in double combination, were supplied for 12 days to growing seedlings of the model species Arabidopsis thaliana. Metal accumulation was measured in roots and shoots. Microscopic analyses revealed that root morphology was affected by metals, and that the root and shoot levels of indole-3-acetic acid, trans-zeatin riboside and dihydrozeatin riboside varied accordingly. Minor modifications in gibberellic acid levels occurred in the Zinc treatments, whereas abscisic acid level did not change after the exposition to metals. Reverse transcription polymerase chain reaction analysis of some genes involved in auxin and cytokinin synthesis (AtAAO, AtNIT and AtIPT) revealed that their expression were not affected by metal treatments. The root morphological alterations that resulted in an increased surface area, due to the formation of root hairs and lateral roots, could be signs of the response to metal stress in terms of a functionally-addressed reorientation of root growth. The root system plasticity observed could be important for better understanding the manner in which the root architecture is shaped by environmental and hormonal stimuli.
Highlights
Plant material and experimental als.[1]
The root uptake of Cd was subjected to a significant competition in the presence of Zn (222 μg/g DW of Cd in the CdSO4 and μM ZnSO4 (Cd/Zn) treatment against 305 μg/g DW of Cd in the CdSO4 and μM CuSO4 (Cd/Cu) treatment) (Table 2)
The similar behavior of Cd was observed for Zn root levels in the presence of Cd (725 μg/g DW of Zn in the CuSO4 and μM ZnSO4 (Cu/Zn) treatment against 461 μg/g DW of Zn in the Cd/Zn treatment (Table 2)
Summary
Plant material and experimental als.[1] The metals were added directly to the Hoagland’s solution. Understanding the metabolic answer and the adaptation of plants towards toxic metal exposure can open the way to future phytoremediation of contaminated sites.[1] In particular, excess cadmium (Cd), copper (Cu) and zinc (Zn) are known to induce stress effects in all plant species.[2] while homeostatic design. Seeds were put in 3 cm-height polyethylene to the metals, roots and shoots were separated and immediately analysed. The 12-day exposure period was chosen on the basis of previous experiments for avoiding toxic metal accumulation in Arabidopsis plants.[1,13] Control plants were not treated with metals
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