Abstract
The aim of the present study was to study spawning stimulation in artificial reproduction of females pikeperch (Sander lucioperca L.) using “Chorulon” containing the human chorionic gonadotropin (hCG) and compare with “Supergestran” containing a mammalian GnRH ([D-Ala6]GnRHProNhet) analogue. The females were divided into eleven experimental groups and injected with hCG at 250, 500, 750, and 1 000 IU kg−1 body weight (BW) and mGnRHa at 1, 2.5, 5, 10, 25, and 50 μg kg−1 BW. In all treatments, a single intramuscular injection of hormone was performed. Control group was injected with 0.9 % NaCl, 0.9 cm3 kg−1 BW. The average percentages of ovulating females were 88.5 ± 12.3 and 80.8 ± 10.9 % in hCG- and mGnRHa-treated groups, respectively. The average diameter of eggs was 0.95 ± 0.06 and 0.98 ± 0.06 mm in hCG- and mGnRHa-treated groups, respectively. Neither ovulation rate nor diameter of egg was statistically differed among hormonally treated groups. Statistical difference was observed only in hatching rate, where the average were 73.6 ± 14.4 and 50.6 ± 17.7 % in hCG and mGnRHa-treated groups, respectively. Among hormonally treated groups, the best results were observed in groups treated with hCG at 500 and 750 IU kg−1 and in groups treated with mGnRHa at 25 μg kg−1. No ovulation was observed in the control group. This study indicated successful ovulation in pikeperch using a single intramuscular injection of hCG or mGnRHa analogue.
Highlights
Pikeperch (Sander lucioperca L.) is a highly valuable commercial fish for inland European aquaculture (Hilge and Steffens 1996), which has an acceptable growth rate to market sizeAquacult Int (2013) 21:811–818 under intensive culture (Fontaine 2009)
The highest ovulation rate (100 %) was observed in females treated with mGnRHa (25 lg kg-1) or with human chorionic gonadotropin (hCG) (500 and 750 IU) (Table 2)
The highest hatching rate was observed in groups stimulated with hCG at 500 and 750 IU
Summary
Pikeperch (Sander lucioperca L.) is a highly valuable commercial fish for inland European aquaculture (Hilge and Steffens 1996), which has an acceptable growth rate to market sizeAquacult Int (2013) 21:811–818 under intensive culture (Fontaine 2009). The stable mass production of fry for open waters or fish farms is not available yet, because the artificial reproduction of pikeperch, reproductive physiology of broodfish, is not still well developed (Philipsen 2008). The studies of optimum methods for artificial reproduction with emphasizes on broodfish reproductive physiology to achieve stable mass production of fry and grow up them to market size are still needed. There are a few studies reporting spawning induction in pikeperch using injection of carp pituitary containing gonadotropins, synthetic analogues of GnRH, human chorionic gonadotropin (hCG), or LHRH-a (Antalfi 1979; Schlumbenger and Proteau 1996; Craig 2000; Kouril and Hamackova 2005; Zakes and Demska-Zakes 2005; Ronyai 2007). The Chorulon has been used for artificial reproduction of African catfish (Clarias gariepinus) (Richter et al 1987; Mollah and Tan 1983), rainbow sharks (Labeo erythrurus) (Shireman and Gildea 1989), redtail black sharks (Labeo bicolor) (Shireman and Gildea 1989), and of pufferfish (Tetraodon nigroviridis) (Watson et al 2009)
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