Hormonal and enzymatic responses of maize seedlings to chilling stress as affected by triazoles seed treatments

Abstract

Triazole fungicides have been used for seed treatment to control soilborne diseases of maize, but seedlings coming from triazole-coated seed show serious phytotoxicity under chilling stress. To understand this phytotoxic impact, maize seed was treated with four triazoles fungicides and the corresponding seedlings were analysed on growth and gene expression. We found that maize seed coated with difenoconazole and tebuconazole exhibited either no or increased effects on germination and growth of maize at 25 °C, regardless of chemical concentrations. When maize seedlings were subjected to chilling treatment, however, their growth was significantly inhibited, and the inhibition was positively correlated with the rate of triazole application. Mesocotyl length decreased by 32.19–44.73% by difenoconazole, and 23.53–32.08% by tebuconazolet at rates of 1:50 and 1:25, respectively. However, myclobutanil did not have any effects at any temperatures. The contents of the gibberellin GA12 and abscisic acid in maize seedlings developed from difenoconazole- or tebuconazole-coated seed were significantly increased under chilling stress. The expression of two key catabolic enzyme genes, GA2ox3 and GA2ox4, was significantly up-regulated immediately following chilling stress and 2 days after recovery at 25 °C in the seedlings treated with difenoconazole or tebuconazole. This imbalance in phytohormones may explain why difenoconazole- or tebuconazole-coated seed more likely results in the phytotoxicity of maize seedlings under a low temperature condition during seed emergence and seedling growth. Since myclobutanil did not have this negative effect, it can be applied for seed coating in areas where temperatures are low during early seedling growth.