Abstract
In order to study the effects of Nosema ceranae infection on honey bee microRNA (miRNA) expression, we deep-sequenced honey bee miRNAs daily across a full 6-day parasite reproduction cycle. Seventeen miRNAs were differentially expressed in honey bees infected by N. ceranae that potentially target over 400 genes predicted to primarily involve ion binding, signaling, the nucleus, transmembrane transport, and DNA binding. Based on Enzyme Code analysis, nine biological pathways were identified by screening target genes against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, seven of which involved metabolism. Our results suggest that differentially expressed miRNAs regulate metabolism related genes of host honey bees in response to N. ceranae infection.
Highlights
Early (1 dpi) and late infection (6 dpi) time points had a higher tendency for differential miRNA expression. This non-random distribution suggests that the honey bee miRNAs we identified might be related to Gene Ontology (GO) Terms Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways
Pyrimidine metabolism and oxidative phosphorylation pathways to be among the predicted functional roles of the miRNA target gene list
N. ceranae spores were isolated from the midguts of worker honey bees in heavily infected colonies and purified using a Percoll gradient procedure[23]
Summary
The purpose of this study is searching the miRNA expression variance due to the infection
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