Abstract
Enzyme immunoassay has been applied to the determination of conjugates of chenodeoxycholate in serum. The procedure does not require extraction of serum nor separation of antibody-bound from free antigen. These two factors reduce the analysis time to less than 5 h, with sensitivity comparable to that of radioimmunoassay. Results correlate well with values determined by radioimmunoassay ( r = 0.99, N = 14) and precision (± 6%, N = 8) compares favourably with reported values for radioimmunoassay.
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