Histological and immunocytochemical characterization of neurons located in the white matter of the spinal cord of the pigeon

Abstract

In the spinal cord of birds a considerable number of neuronal somata is located outside the gray matter. Some of these neurons form segmental marginal nuclei, which lie at the border of the spinal cord near the dentate ligament. In lumbosacral segments these marginal nuclei form accessory lobes which bulge into the vertebral canal. These lobes consist in neurons which are embedded into glia-derived glycogen cells. Furthermore, there are neurons in the white matter near the accessory lobes and numerous paragriseal cells lying in the lateral and ventral funiculus. Glycogen cells are present both in the lobes and in the glycogen body which fills the lumbosacral spinal rhomboid sinus. Immunoreactivity of glial fibrillary acidic protein, a marker of astrocytes, was used to characterize the surrounding of marginal neurons. Astrocytes were numerous in cervical marginal nuclei but rare in accessory lobes. There is cytological (distribution of Nissl substance) and immunocytochemical evidence (immunoreactivity of medium-sized neurofilament, glutamic acid decorboxylase and glutamatergic AMPA receptor subtype GluR2/3) that neurons of the accessory lobes and the nearby white matter are similar, whereas paragriseal cells are different.