Abstract
Cyanogenic glycosides are present in several economically important plant foods. Amygdalin, one of the most common cyanoglucoside, can be found abundantly in the seeds of apples, bitter almonds, apricots, peaches, various beans, cereals, cassava and sorghum. Amygdalin has been used for the treatment of cancer, it shows killing effects on cancer cells by release of cyanide. However, its effect on bone structure has not been investigated to date. Therefore, the objective of this study was to determine a possible effect of amygdalin application on femoral bone microstructure in adult rabbits. Four month old rabbits were randomly divided into two groups of three animals each. Rabbits from E group received amygdalin intramuscularly at a dose 0.6 mg.kg-1 body weight (bw) (group E, n = 3) one time per day during 28 days. The second group of rabbits without amygdalin supplementation served as a control (group C, n = 3). After 28 days, histological structure of femoral bones in both groups of rabbits was analysed and compared. Rabbits from E group displayed different microstructure in middle part of the compact bone and near endosteal bone surface. For endosteal border, an absence of the primary vascular longitudinal bone tissue was typical. This part of the bone was formed by irregular Haversian and/or by dense Haversian bone tissues. In the middle part of substantia compacta, primary vascular longitudinal bone tissue was observed. Cortical bone thickness did not change between rabbits from E and C groups. However, rabbits from E group had a significantly lower values of primary osteons' vascular canals and secondary osteons as compared to the C group. On the other hand, all measured parameters of Haversian canals did not differ between rabbits from both groups. Our results demonstrate that intramuscular application of amygdalin at the dose used in our study affects femoral bone microstructure in rabbits.
Highlights
Cyanogenic glycosides are natural plant toxicants (Bolarinwa et al, 2015)
The middle part of the compact bone was composed by dense Haversian bone tissue with a high concentration of secondary osteons
Our results demonstrate that administration of amygdalin at the dose 0.6 mg.kg-1 bw one time per day during 28 days induced changes in femoral bone microstructure of rabbits
Summary
Cyanogenic glycosides are natural plant toxicants (Bolarinwa et al, 2015). All cyanogenic glycosides are potentially dangerous due to production of hydrogen cyanide (HCN) by their hydrolysis (Vetter, 2000), known as prussic acid (Francisco and Pinotti, 2000).Consumption of cyanogenic plants may cause acute and chronic toxicity in both animals and humans (Yildirim and Askin, 2010).Amygdalin, D-mandelonitrile-β-D-gentiobioside, C20H27NO11, is one of the most frequently occurring cyanogenic glycosides (Bolarinwa et al, 2014). Cyanogenic glycosides are natural plant toxicants (Bolarinwa et al, 2015). All cyanogenic glycosides are potentially dangerous due to production of hydrogen cyanide (HCN) by their hydrolysis (Vetter, 2000), known as prussic acid (Francisco and Pinotti, 2000). Consumption of cyanogenic plants may cause acute and chronic toxicity in both animals and humans (Yildirim and Askin, 2010). D-mandelonitrile-β-D-gentiobioside, C20H27NO11, is one of the most frequently occurring cyanogenic glycosides (Bolarinwa et al, 2014). It is especially presented in fruit kernels of peaches, apricots, bitter almonds (Blaheta et al, 2016), pears, plums and apples (Kolesár et al, 2015). Amygdalin is composed of two molecules of glucose, one of benzaldehyde and one of hydrocyanic acid (Chang et al, 2006; Abdel-Rahman, 2011)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
