Abstract
Various neurobiological evidence indicates that A-subtype cholecystokinin (CCK A) receptors are widely distributed through the mammalian neuroaxis despite the sparse localization found by receptor autoradiography. To address this paradox, immunohistochemistry has been performed in rat brain and spinal cord using an antibody directed at a portion of the amino terminal sequence of the CCK A receptor. Immunoreactivity, visualised using diaminobenzidine, was widely and topographically distributed being most concentrated in medulla and spinal cord. Many forebrain areas contained specifically labelled neurones, notably the nucleus accumbens, septum, stria terminalis, habenula, substantia nigra, ventral tegmental area and lateral geniculate nucleus. In medulla, heavily labelled perikarya were found in parabrachial and trigeminal nuclei, while in spinal cord immunoreactivity was localized in dorsal horn. Localization of immunoreactivity was consistent with the reported distribution of CCK A receptor-mediated mechanisms. Our observations represent the first attempt to describe the localization of the CCK A receptor in brain using immunohistochemistry and support its wide functional involvement in the central nervous system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
