High-sensitive detection of okadaic acid using human bronchial epithelial cell-based impedance biosensor

Abstract

Okadaic acid (OA) is typical diarrhetic shellfish poisoning (DSP) toxins, and many detection methods were established for its detection. However, the drawbacks of these methods hamper their wide applications. Here, human bronchial epithelial cell lines (BEAS2B) were selected as the sensitive elements to fabricate the high-sensitive cell-based impedance biosensor to detect the cytotoxicity induced by okadaic acid (OA). The half maximal inhibitory concentration (IC50) of BEAS2B-based impedance biosensors for OA detection at 24 h and 96 h were 4.10 ± 1.04 nM (3.30 ± 0.84 μg/L) and 1.62 ± 1.07 nM (1.30 ± 0.86 μg/L), respectively, which are both significantly lower than those of human cancer cell-based impedance biosensors. Besides, the specificity of the BEAS2B-based impedance biosensors were tested by the microcystin (MC). All the results indicate that our high-sensitive human bronchial epithelial cell-based impedance biosensor will be an alternative tool in the OA assessment.