Abstract
Potato mop-top virus (PMTV) is the type member of the genus Pomovirus characterized by a tripartite, single-stranded, positive-sense RNA genome. However, PMTV differs from the other pomoviruses by having an open reading frame (ORF) for a 8-kDa cysteine-rich protein (CRP) on RNA 3, downstream the triple gene block. Site-directed mutagenesis of infectious cDNA clones of PMTV showed that the CRP ORF is not needed for the systemic infection in Nicotiana benthamiana and several other hosts, but it has an effect on the symptom development of PMTV. CRP of PMTV was not capable of RNA silencing suppression in agroinfiltration tests. However, the expression of CRP using vectors based on Potato virus X (PVX) or Tobacco mosaic virus (TMV) resulted in necrotic symptoms on N. benthamiana and tobacco plants, respectively. Sequencing of PMTV isolates obtained from the field revealed significant sequence variability of the CRP. These data suggest that the functions of CRP may be expressed only in certain hosts or at certain phases of the PMTV infection cycle.
Highlights
Over the past two decades, it has been recognized that nitric oxide (NO) plays an important role in diverse mammalian physiological processes
The increase of leaf flavonoid content can be a protection against the harmful effect of UV-B radiation as they function as antioxidant and UV filters in plants
The results demonstrate that Nitric oxide (NO) is produced in plant tissues grown under low oxygen tensions and suggest that class 1 hemoglobins have a significant function in regulating NO levels
Summary
Microtubules provide structural support for cells and play a key role in mitosis and meiosis. More detailed analysis of reproductive organs development of IPC-resistant mutants revealed that microspores in anthers are subjected to degenerative process, leading to pollen sterility. It was found the chromatin agglutination in nuclei of most part of microsporocytes. Summarising obtained results we can consider that all established abnormalities in male reproductive organ development leading to PCD in pollen production can be arise from a mutation of the microtubule protein(s) in IPC-resistant N. sylvestris lines. The analysis of IPC-resistant N. sylvestris plants is continuing to determine the role of certain mutant cytoskeletal gene(s) which is/are express(es) in male gametophyte and lead to PCD during pollen production for better understanding the male reproductive organ development.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
