Abstract
Laser scanning microscopy is playing a major role in visualization of biological structures and processes. However, as images are degraded due to blurring, noise, and color shifts, quantitative interpretation of confocal images can be difficult. In this chapter, we detail a procedure that involves acquisition of high-resolution confocal image stacks in tissue sections and the subsequent deconvolution process. Data generated using these methods can be used for reliable quantification of cell biological and tissue interactions, e.g., colocalization analyses or 3D reconstructions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.