Abstract
A high-performance liquid chromatography tandem mass spectrometry-mass spectrometry (LC-MS-MS) assay was developed for the analyses of morphine, morphine glucuronides and normorphine in plasma samples from rats. The analytes were extracted by using C 2 solid-phase extraction cartridges. The extraction recoveries were 100% for morphine, 84% for morphine-3-glucuronide, 64% for morphine-6-glucuronide and 88% for normorphine. Both intra- and inter-assay variabilities were below 11%. Using a plasma sample size of 100 μl, the limits of detection were 13 nmol l −1 (3.8 ng ml −1) for morphine, 12 nmol l −1 (5.5 ng ml −1) for morphine-3-glucuronide, 26 nmol l −1 (12 ng ml −1) for morphine-6-glucuronide and 18 nmol l −1 (5.0 ng ml −1) for normorphine, at a signal-to-noise ratio of 3. The present assay was applied to a pharmacokinetic study in rats after intraperitoneal administration of morphine.
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