Abstract
Abstract Despite recent findings for the treatment of multiple myeloma (MM) it still remains a deadly disease. In the effort to develop new and more effective therapies for human tumors, murine animal models have been proven critical for a variety of neoplasias. Unfortunately, although recent studies have described different animal models of human MM, none is available for tracking the tumor progression with a specific biomarker employing human MM cells. This lack hampers the development of new approaches for treating MM. Here we describe a human MM model in NOD/SCID mice that allows for highly sensitive and specific monitoring of disease progression through the endogenously-expressed tumor specific cancer/testis antigen AKAP-4. The human MM cells U266 were subcutaneously injected in NOD/SCID animals. AKAP-4 expression resulted to be a reliable and sensitive antigen to monitor tumor growth and spread by different and independent techniques, namely flow-cytometry, E.L.I.S.A., Western blot and RT-PCR. The relevance of our findings stems from the suitability of this innovative model for a thorough pre-clinical evaluation of experimental pharmacological strategies for the treatment of human MM.
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