Abstract
In this study, an electrochemical sensor was fabricated for detecting aflatoxin B1 (AFB1) based on in situ-initiated ring-opening metathesis polymerization. The thiol-modified aptamer was first immobilized on the Au electrode through Au-S bonding to capture AFB1. Then, the acrylic acid (AA)-functionalized antibody specifically recognized AFB1 and immobilized on the surface of the electrode. Finally, a large number of cyclopentenyl ferrocene (CFc) monomers were grafted onto the electrode surface under the action of the catalyst AquaMet, forming an electroactive long-chain polymer that results in a significant signal enhancement. Under optimal conditions, the detection limit of the method was 0.34 fg/mL with a detection range of 2 fg/mL-2 ng/mL. The proposed sensor is highly selective due to the high specificity of the aptamer and antibody to the target. In addition, the sensor has been successfully applied to detect AFB1 in real samples with recoveries ranging from 92.91% to 107.20%.
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