Abstract
Summary In order to explore transgenic plants for large-scale production of pharmaceutical proteins as recombinant antibodies, we tried to optimize the accumulation and stability of functionally active single-chain Fv antibodies in transgenic tobacco plants. Two different single-chain Fv antibodies were used for our studies, which were expressed in different plant organs and plant cell compartments. For ubiquitous expression in tobacco plants transcription of the single-chain Fv genes was controlled by the strong CaMV 35S promoter. For storage-organ-specific expression we used two seed-specific Vicia faba promoters from the legumin B4 and the unknown seed protein gene. Summing up our results plant expression systems where the recombinant antibody fragments are retained in the endoplasmic reticulum provide not only high accumulation levels of active antibodies in leaves and seeds but also allow short- and long-term storage of highly active immunoglobulins in harvested plant material at room temperature.
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