Abstract
Studies of the molecular mechanisms involved in the formation of the albino leaf cells are important for understanding the development of chimera leaves in Ananas comosus var. bracteatus. In this study, we identified a total of 163 novel miRNAs involved in the development of complete white (CWh) and complete green (CGr) leaves using high-throughput sequencing method. The potential miRNA target genes were predicted and annotated using the NR, Swiss-Prot, GO, COG, KEGG, KOG and Pfam databases. The main biological processes regulated by miRNAs were revealed. The miRNAs which potentially play important roles in the development of the leaves and the albino of the CWh leaf cells were selected and their expression patterns were analyzed. The expression levels of nine miRNAs and their potential target genes were studied using qRT-PCR. These results will help to elucidate the functional and regulatory roles of miRNAs in the formation of the albino cells and the development of the leaves of A. comosus var. bracteatus. These data may also be helpful as a resource for studies of small RNA in other leaf color chimeric plant species.
Highlights
IntroductionAnanas comosus var. bracteatus (red pineapple) is a commercially cultivated monocot plant originating from South America
Ananas comosus var. bracteatus is a commercially cultivated monocot plant originating from South America
In order to identify miRNAs and to reveal the regulation mechanisms of gene expression in the A. comosus var. bracteatus leaves, the high-throughput sequencing of six miRNA libraries constructed from the complete green (CGr) and complete white (CWh) leaves at three developmental stages were performed
Summary
Ananas comosus var. bracteatus (red pineapple) is a commercially cultivated monocot plant originating from South America. The results of transcriptome sequence revealed the significant differences in transcription levels between the CGr and CWh plants, especially in those aspects that control photosynthesis, porphyrin and chlorophyll metabolism, and carotenoid biosynthesis[9]. These studies provided a frame of transcription variations between CGr and CWh plants that most likely result in the formation of the albino leaf cells. Based on these results, we could further study the regulatory roles of small RNA presumably involved in the development of albino leaves. Our results provide a valuable resource for studying microRNA regulation involved in the leaf color formation and leaf development of A. comosus var. bracteatus
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