Abstract
We describe a high-throughput hyperspectral microscope. The system replaces the slit of conventional pushbroom spectral imagers with a static coded aperture mask. We present the theoretical underpinnings of the aperture coded spectral engine and describe two proof-of-concept experimental implementations. Compared to a conventional pushbroom system, the aperture coded systems have 32 times greater throughput. Both systems have about a 1 nm spectral resolution over the spectral range of 550-665 nm. For the first design, the spatial resolution for the system is 5.4 microm while the spatial resolution for the second system ranges from 7.7 microm to 1.54 microm. We describe experimental results from proof-of-concept applications of the imager to hyperspectral microscopy.
Highlights
In this paper, we describe the use of static mask coded-aperture spectroscopy to increase photon efficiency in hyperspectral microscopy (HM)
We describe experimental results from proof-of-concept applications of the imager to hyperspectral microscopy
We describe the use of static mask coded-aperture spectroscopy to increase photon efficiency in hyperspectral microscopy (HM)
Summary
We describe the use of static mask coded-aperture spectroscopy to increase photon efficiency in hyperspectral microscopy (HM). A given spatio-spectral element tends to contain very few photons and has a poor signal-to-noise ratio (SNR) In response to this problem, the hyperspectral imaging community developed a number of different direct-view designs that maximize the light gathering efficiency of the systems [5, 6, 7]. These systems do away with the spectrometer slit altogether and view the source through a rotating dispersive element. We present experimental results from two proof-of-concept hyperspectral microscopes (HM) that we have constructed with these techniques
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