Abstract
Trophozoites or the free-swimming form of the intestinal protozoan,Giardia, resides within the host small intestine where they attach to the microvillous border by means of a grasping organelle, the ventral adhesive disc. Several mechanisms have been proposed to explain trophozoite attachment including contractile activity, microtubular coiling-uncoiling, hydryodynamic activity of the ventral flagella, and activated lectin binding.We have developed a model for investigating the mechanism of attachment ofGiardiatrophozoites to the microvillous border of cultured intestinal epithelial cells. Caco-2 cells, a human colonic intestinal cell line was grown for 14 days on glass cover slips in Delbeccos' modified Eagle's medium.Giardiatrophozoites (Be-1;IP-0482:1) were cultured in TYI medium for 3 days, then harvested and resuspended in modified Eagle's medium prior to incubation for 30 minutes on Caco-2 cells. Coverslips were rinsed with warm Hanks balanced salt solution, fixed overnight in 3% glutaraldehye - 0.1 M cacodylate buffer, pH 7.2, dehydrated in ethanol, and critical point dried in CO2.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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