Abstract
ABSTRACTBurkholderia contaminans strain CH-1 converts 2-azahypoxnathine to 2-aza-8-oxohypoxanthine, plant growth-regulating compounds, by oxidation. We report here the high-quality draft genome sequence of B. contaminans CH-1. The genome contains 8,065 protein-coding sequences, including several genes possibly involved in metabolizing 2-azahypoxanthine.
Highlights
Burkholderia contaminans belongs to the Burkholderia cepacia complex, which comprises approximately 20 closely related species [1]
To construct scaffolds of the CH-1 genome, the contigs were aligned to the LMG 23361 genome sequence using CONTIGuator version 2.7 [8]
Among 8,065 proteins, 82 were assigned to the purine metabolism pathway, including the xanthine dehydrogenase small subunit (XdhA, K13481) and large subunit (XdhB, K13482), which are possibly involved in the conversion of AHX to AOH, as predicted by the purine metabolism pathway in plants [14]
Summary
Burkholderia contaminans belongs to the Burkholderia cepacia complex, which comprises approximately 20 closely related species [1]. Genomic DNA of B. contaminans strain CH-1 was extracted using the DNeasy blood and tissue kit and fragmented using the Covaris acoustic solubilizer. A paired-end library constructed by the TruSeq DNA PCR-free library preparation kit was sequenced using the Illumina MiSeq platform (301-bp paired-end) at the Instrumental Research Support Office, Research Institute of Green Science and Technology, Shizuoka University.
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