Abstract
An ion-pair reversed-phase high-performance liquid chromatographic (HPLC) method for the separation and simultaneous determination of thiamine HCl (vitamin B1), riboflavin (vitamin B2), nicotinamide, pyridoxine HCl (vitamin B6), cyanocobalamin (vitamin B12), ascorbic acid (vitamin C) and folic acid in capsule preparations is described. The above seven active substances were extracted from the preparations within 5 min using an electronically controlled extraction apparatus. Ion-pair reversed-phase HPLC was carried out on LiChrosorb RP-18 using methanol-water-concentrated phosphoric acid-octanesulphonic acid as the eluent. All seven active ingredients were separated in less than 4 min. The detection limits were 1–2 ng for all vitamins; however, amounts of 5–10 ng were required for the quantitative HPLC determination of the vitamins. The proposed HPLC method is suitable for the determination of these active ingredients in pharmaceutical preparations such as multivitamin capsules and has a coefficient of variation between 1.1 and 3.2%. The ease of extraction, accuracy and sensitivity of the method and the time required for analysis are emphasised.
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