Abstract
A high-performance liquid chromatographic method of analysing hyaluronic acid and oligosaccharides produced by bovine testes hyaluronidase is presented. Using silica gel packed columns and an aqueous mobile phase, it is possible to separate the mono-, di-, tetra- and hexasaccharides from higher oligosaccharides and hyaluronic acid and to follow the enzymatic conversion of polysaccharides to oligosaccharides. The analysis is performed within 30 min and only 20 μl of sample (containing 20–60 μg of saccharides) are needed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
