Abstract
High-performance liquid chromatography (HPLC) coupled to nuclear magnetic resonance (NMR) spectroscopy has been used to obtain 1H NMR spectra of ecdysteroids present in an extract of the plant Silene otites. Reversed-phase gradient chromatography was performed using a 2H 2O–acetonitrile–trifluoroacetic acid-based solvent system. Diagnostic NMR spectra were obtained for 20-hydroxyecdysone, 2-deoxy-20-hydroxyecdysone and 2-deoxyecdysone using the stopped-flow HPLC–NMR technique. The compounds were present in the extracts in amounts between ca. 12 and 135 μg on-column (as determined using HPLC–UV).
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