Abstract

A high-performance liquid chromatographic method has been developed for the separation of chlorhexidine and its known degradation product, p-chloroaniline. These amine-containing compounds can be separated without the addition of ion-pairing reagents and/or amine modifiers if the proper specialty column is selected. A photodiode-array detector was used to acquire spectral data and demonstrate the importance of the mobile phase pH when optimizing the response of p-chloroaniline.

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