Abstract

1. 1. Hemolystate from heavily stressed smooth hammerhead shark, Sphyrna zygaena, shows three electrophoretic components, SZ I, SZ II and SZ III, whose relative concentrations are 36.4 ± 6.8, 36.4 ± 5.0 and 20.8 ± 5.7%, respectively. After reduction with DTE only SZ I remained. 2. 2. SZ I reacted with glutathione disulfide reconstitute SZ II and SZ III. 3. 3. Non-reduced, DTE-reduced, and denatured hemoglobin were found to have 2.0 ± 0.4, 3.7 ± 0.6, and 9.4 ± 0.7-SH groups, respectively. 4. 4. Erythrocyte non-protein—SH (NPSH), including glutathione present as mixed disulfide with SZ II and SZ III, is 1.7 NPSH/Hb.

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