High-frequency multiple shoot regeneration from cotyledonary nodes of guar (Cyamopsis tetragonoloba L. Taub)

Abstract

Guar (Cyamopsis tetragonoloba L. Taub) is a drought-tolerant multipurpose cash crop. A rapid regeneration system has been developed for four Indian guar genotypes. Investigations were carried out to assess the effect of different growth regulators and their combinations on a variety of explants such as the embryo, cotyledons, and cotyledonary nodes for shoot morphogenesis. It was established that Murashige and Skoog's culture medium containing 6-benzylaminopurine (13.3 μM or 3 mgl−1) in combination with indole-3-acetic acid (11.4 μM or 2mgl−1) with cotyledonary node explants gave the highest frequency of multiple shoot induction. In vitro rooting from cultured shoots was maximal on a half-salt concentration of Murashige and Skoog's culture medium fortified with indole-3-butyric acid (4.9 μM or 1 mgl−1). In vitro-regenerated plants were grown to pod setting and subsequent maturity in greenhouse conditions.