Abstract
31P NMR measurements have been made of both in vivo cell suspension cultures and cell extracts of Nicotiana tabacum. Comparison of the spectra with those of reference compounds and pH variation studies on the extracts has allowed signals attributable to inorganic phosphate, glucose-6-phosphate, sugar phosphates, phosphodiester, ATP, ADP and a nucleoside-diphosphoglucose to be identified. The in vivo study has allowed most of these signals to be monitored throughout the growth cycle and hence changes in the composition of the cytoplasm and vacuole have been followed. ATP and ADP are both present as the Mg2+-complex in the cytoplasm.
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