Abstract
The fabrication and testing of microfluidic spinning compact discs with embedded trapezoidal microchambers for the purpose of inertial microparticle focusing is reported in this article. Microparticle focusing channels require small features that cannot be easily fabricated in acrylic sheets and are complicated to realize in glass by traditional lithography techniques; therefore, the fabrication of microfluidic discs with femtosecond laser ablation is reported for the first time in this paper. It could be demonstrated that high-efficiency inertial focusing of 5 and 10 µm particles is achieved in a channel with trapezoidal microchambers regardless of the direction of disc rotation, which correlates to the dominance of inertial forces over Coriolis forces. To achieve the highest throughput possible, the suspension concentration was increased from 0.001% (w/v) to 0.005% (w/v). The focusing efficiency was 98.7% for the 10 µm particles and 93.75% for the 5 µm particles.
Highlights
Particle/cell separation of biological samples is a crucial step in sample preparation for various biomedical assays
We study microfluidic discs with straight channels and integrated trapezoidal
After fixing a microfluidic disc on the spinning system platform and injecting the particle solution into the source chamber, the disc’s rotational speed must exceed the burst frequency so that the solution can flow in the focusing channel
Summary
Particle/cell separation of biological samples is a crucial step in sample preparation for various biomedical assays. The flow inside microchannels can be induced in two main methods; one is by using an external pump, such as a syringe pump or an high performance liquid chromatography (HPLC) pump [8], while another is utilizing centrifugal forces in a disc-shaped platform In such a design, microfluidic features, known as “chambers”, contain the sample fluid, while other features, known as “channels”, connect different chambers and act as a passage for fluids inside the disc. Passive methods in general do not require an external force to isolate particles; implementing inertial separation on microfluidic discs should be very simple to operate Such a method has proven to be a simple and efficient way to produce high purity blood plasma [29]. Glass fabrication can cost more than PMMA and PDMS as it requires access to femtosecond laser machining and a cleanroom
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