Hg and metallothionein-like proteins in the black scabbardfish Aphanopus carbo

Abstract

Aphanopus carbo fishery is economically relevant in Portugal (Madeira Archipelago) since the 19th century and an important diet component of its inhabitants. However, Hg levels in this species are a cause of concern due to the insufficient knowledge of Hg subcellular distribution, accumulation and detoxification mechanisms. Hg concentrations (total and subcellular) and metallothionein-like proteins (MTs) were measured in the gills, muscle and liver of A. carbo of different size (50–150 cm) to assess Hg subcellular distribution and the role of MT in Hg binding. Total Hg concentrations in A. carbo decrease from liver to gills and increase exponentially with fish length in all tissues. Hg subcellular distribution in muscle and liver decreased from insoluble > high molecular weight proteins and heat-treated cytosol where MT occurs highlighting the role of the insoluble fraction in Hg distribution. In the gills, Hg was mainly in the soluble fraction except for 75–100 cm specimens, whose Hg distribution was similar between soluble and insoluble factions. The variation of MT with size is different from that of Hg. MT levels in the gills decrease with size/age and is unrelated with Hg concentrations. In the muscle although decreasing with size/age, MT is inversely proportional to Hg. In the liver, however, MT is directly proportional with size/age and to the Hg accumulated (total and HTC subcellular fraction) highlighting an age related change in these two parameters and the importance of MT in detoxifying Hg in this tissue. Due to Hg concentrations present in the muscle (used for human consumption) and liver (consumed mainly by fisherman) consumption of A. carbo should be taken with caution in a population with such an important seafood weekly intake.