Abstract
Cell culture is an important research method in virology. Although many attempts were tried to culture HEV in cells, only two cell culture systems were considered to have high enough efficient for usage. Concentration of virus stocks, host cells, and medium components affect the culture efficient, and the genetic mutations during HEV passage were found to be associated with the increased virulence in cell culture. As an alternative method for traditional cell culture, the infectious cDNA clones were constructed. The viral thermal stability, factors that impact the host range, posttranslation of viral proteins and function of different viral protein were studied using the infectious cDNA clones. The studies on progeny virus showed that the virus secreted from host cells have an envelope, and its formation was associated with pORF3. This result explained the phenomenon that virus could infect hosts cells in the presence of anti-HEV antibodies.
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