Abstract

A strain YZN-001 was isolated from swine manure effluent and was identified as Pseudomonas stutzeri. It can utilise not only nitrate and nitrite, but also ammonium. The strain had the capability to fully remove as much as 275.08mg L−1 NO3−–N and 171.40mgL−1 NO2−–N under aerobic conditions. Furthermore, At 30°C, the utilization of ammonium is approximately 95% by 18h with a similar level removed by 72h and 2 weeks at 10 and 4°C, respectively. Triplicate sets of tightly sealed serum bottles were used to test the heterotrophic nitrifying ability of P. stutzeri YZN-001. The results showing that 39% of removed NH4+–N was completely oxidised to nitrogen gas by 18h. Indicating that the strain has heterotrophic nitrification and aerobic denitrification abilities, with the notable ability to remove ammonium at low temperatures, demonstrating a potential using the strain for future application in waste water treatment.

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