Abstract
ABSTRACT Currently, the knowledge of ecology and function of bacteria harbored by molluscs is very limited. In this study, quantity and richness of culturable heterotrophic bacteria (CHB) in seawater of the farming environment and guts of the oyster Crassostrea hongkongensis were determined monthly, with classical, biochemical, and molecular methods. About 800 randomly selected bacterial strains were purified and identified.Quantities of CHB and vibrios, ranging from2.3×103 to 2.2×105 CFU/ml and 1.6×102 to 3.0×103 CFU/ml, respectively, were present in the farming seawater, whereas those in the oyster C. hongkongensis ranged from 5.3×104 to 5.4×106 CFU/g and 6.0×103 to 1.4×105 CFU/g, respectively. Vibrio spp., Pseudoalteromonas spp., Bacillus spp., and Shewanella spp. appeared almost every month. Richness of heterotrophic microbial communities of the seawater and the oysters was very similar and peaked in August. Variation in the diversity (Shannon index) of CHB in the seawater and in the oysters was simil...
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