Abstract
An analysis has been made of some of the factors influencing the uptake of 3H-labeled Escherichia coli deoxyribonucleic acid (DNA) by mice fibroblasts (NCTC cells) in vitro: DNA concentration, incubation time, pH and temperature of the medium, sonication and denaturation of the DNA influence the quantitative uptake of 3H-labelled material. This uptake is reduced by metabolic inhibitors (2,4,dinitrophenol, cyanide, arseniate). The DNA penetrates into the cell nucleus, and a CsCl gradient centrifugation analysis suggests that exogenous DNA penetrates into the cells without being degraded, retaining its double-stranded form during the first step of penetration. On subsequent incubation, the disappearance of tritiated DNA of E. coli characterized by its density corresponds to a labelling increase into cellular DNA.
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