Hepato-Renal Protective Effects of Mangosteen (Garcinia mangostana L.) Pericarp Extract in Streptozotocin-induced Diabetic Mice

Abstract

This study aimed to explore the effect of mangosteen pericarp extract used for reduce random blood glucose, total cholesterol, SGOT, SGPT, plasma creatinine levels, also ameliorates damaged liver hepatocytes and renal proximal tubular cells in diabetic mice. In this study, we used male mice (Mus musculus) of the BALB/C strain which were divided into 2 groups: the control group (without treatment of mangosteen pericarp extract) and the treatment group. The control group was divided into three: normal control (KN), diabetic control (KD), diabetic control-Metformin HCl (KM). The treatment group (with mangosteen pericarp extract) was divided into 3 groups (P1, P2, and P3) with the dose of 50 mg/kg body weight, 100 mg/kg body weight, and 200 mg/kg body weight, respectively. The induction of diabetes was done with the injection of multiple low-doses of STZ (30 mg/kg of body weight) for 5 consecutive days. Before and after STZ injection, random blood glucose and total cholesterol were measured at 1st, 7th, and 14th day of mangosteen pericarp extract treatments. Treatments were given for 14 days. At 15th day, SGOT, SGPT and plasma creatinine levels were measured using Pentra C200, while liver and kidney were collected and then processed into histological slides. Interestingly, we found that mangosteen pericarp extract administration was able to reduce random blood glucose, total cholesterol, SGOT, SGPT, plasma creatinine levels, also ameliorates damaged liver hepatocytes and renal proximal tubular cells in diabetic mice significantly. In conclusion, mangosteen pericarp extract is a promising antidiabetic agent due to its anti-hyperglycemic and antioxidant properties.