Hepatic oxidative stress markers in rats exposed to silver nanoparticles

The Effectiveness of a Human Patient Simulator in the ATLS Shock Skills Station

Objectives • To assess the intensity of labor pain and outcome of labor among the control group. • To assess the effectiveness of ambulation on intensity of labor pain and outcome of labor among the experimental group. • To assess the usefulness of ambulation by opinionnaire among experimental group. • To compare the effectiveness of ambulation on the intensity of labor pain and outcome of labor among experimental and control groups. • To find an association between the intensity of labor pain with selected demographic variables. Materials and methods An experimental research approach was used for the study. Random sampling technique was used to allocate the subjects into experimental and control groups. Visual analog scale was used to assess the intensity of pain and observational check list was used to assess the outcome of labor. The study comprised of 40 primigravida mothers and they were grouped as experimental and control through randomization. One group received ambulation treatment, the other did not. The researcher then observed the groups to determine the effect of the treatment. Results The collected data was analyzed by descriptive and inferential statistics. The intensity of pain revealed that 40% in the experimental and 55% in the control groups experienced severe pain. The labor augmentation revealed that both oxytocin and cerviprime gel was used for 30% in the experimental and 35% in the control groups. On the mode of delivery, majority of mothers (60%) had normal vaginal delivery both in experimental and control groups. On the duration of the first stage of labor revealed that 50% in the experimental and 20% in the control groups were between 10 to 12 hours. Second stage of labor revealed that majority were between 1 and 2 hours in both the control and experimental groups. The opinion of experimental group mothers on ambulation revealed that half of the mothers (50%) agreed for recommending the ambulation for their neighbors and friends and less than half (35%) of them have agreed for comfort during first stage of labor and 40% of them have agreed for ambulation should be made as a routine in labor room. In the experimental group, mean pain score (6.8) is less than the control group (7.5). The calculated Mann-Whitney Z-value (2.045) is greater than the Z α-value of 1.960 at 0.05 level of significance. In the experimental group, the mean duration of first stage of labor score (3.95) is significantly higher than the control group mean score (3.00). The mean outcome of labor in experimental group (13.95) is greater than the control group. Conclusion There was a significant difference in the duration of first stage of labor between the experimental and control groups. But, there was no significant difference in the overall outcome of labor between experimental and control groups. There was no significant association between intensity of labor pain with selected demographic variables. This may be because of small sample size. How to cite this article Savitha V, Nayak S, Paul S. Effect of Ambulation during First Stage of Labor on Labor Pain and Outcome of Labor among the Primigravida Mothers in a Selected Hospital, Mangalore. J South Asian Feder Obst Gynae 2013;5(1):1-3.

SummaryBackgroundTo assess multimodal imaging features of medication-related osteonecrosis of the jaw (MRONJ) and to analyze the differences between oral and parenteral routes of medication administration. We retrospectively reviewed panoramic radiographs, CT, MRI, and bone scintigraphy of patients with MRONJ.Material/MethodsA retrospective study was conducted in 16 patients with MRONJ who underwent panoramic radiography, CT, MRI, and bone scintigraphy. Statistical analysis for the comparison between routes of medication administration and multimodal imaging features was performed with the Pearson’s χ2 test.ResultsThe percentage of cases with sequestrum separation was 25.0% (4/16 cases) on panoramic radiography and 81.3% (13/16 cases) on CT. The percentage of cases with periosteal bone proliferation on CT was 41.7% (5/12 cases) in the oral route of administration vs. 100% (4/4 cases) in the parenteral route of administration (p=0.042). The percentage of cases with spread of soft tissue inflammation to buccal and other spaces on CT and MRI was 33.3% (4/12 cases) in the oral route of administration vs. 100% (4/4 cases) in the parenteral route of administration (p=0.021).ConclusionsThe sequestrum separation on panoramic radiography in patients with MRONJ was unclear in comparison to CT. Furthermore, characteristic CT findings of patients with MRONJ in the parenteral administration group were periosteal bone proliferation and spread of soft tissue inflammation to buccal and other spaces.

Zmiany metylacji i oksydacji w próbkach DNA bydła bytującego w środowisku zanieczyszczonym metalami ciężkimi

Objective To investigate the effects of probiotics on the survival of sepsis mice and their mechanism. Methods A total of 60 male C57BL6 mice were randomly divided into 3 groups, respectively, the sham operation group (n = 20), the control group (n = 20) and the experimental group (n = 20). After feeding 2 d, mice in the experimental group were given 200 μL probiotics solution daily; mice in the control and sham operation groups were given 200 μL NaCl solution, with continuous intragastric administration for 4 weeks. Cecal ligation and puncture (CLP) was performed on the mice in the experimental and control groups, while mice in the sham operation group were performed with the same procedures of CLP except for cecal puncture. Ten mice in each group were used to observe their activity and survival of 7 d, and the blood and colon tissues were taken in the other 10 mice in each group after 24 hours. The expression levels of serum inflammatory factor interleukin 22 (IL-22), IL-2 and tumor necrosis factor alpha (TNF-α) were detected by enzyme-linked immunosorbent assay, the colon tissues were measured by the hematoxylin and eosin (HE) staining method, and the expression of colonic mucosa (Occludin) in each group was observed by the immunohistochemical method. Results Mice in the sham operation group grew well, mice in the control group curled up in a corner of the cage and trembled, and mice in the experimental group were more active than the experimental group, without obvious tremor. At 7 d, there were still 3 survived mice in the experimental group which were given euthanasia; the 7 d survival was significantly higher than that of the control group (P = 0.020). At 7 d, 10 mice in the sham operation group all survived and were given euthanasia. The expressions of serum IL-22 [(103 ± 23) ng/L vs. (27 ± 9) ng/L, t = 7.590, P < 0.001], IL-2 [(328 ± 27) ng/L vs. (77 ± 21) ng/L, t = 21.368, P < 0.001] and TNF-α [(94 ± 22) ng/L vs. (56 ± 9) ng/L, t = 4.734, P < 0.001] in the control group were significantly different as compared to the sham operation group. Meanwhile, the expressions of serum IL-22 [(75 ± 33) ng/L vs. (27 ± 9) ng/L, t = 3.755, P = 0.001], IL-2 [(217 ± 30) ng/L vs. (77 ± 21) ng/L, t = 10.850, P < 0.001] and TNF-α [(107 ± 20) ng/L vs. (56 ± 9) ng/L, t = 5.956, P < 0.001] in the experimental and sham operation groups all showed statistically significant differences. Compared with the control group, the expressions of serum IL-22 [(103 ± 23) ng/L vs. (75 ± 33) ng/L, t = 2.185, P = 0.042] and IL-2 [(328 ± 27) ng/L vs. (217 ± 30) ng/L, t = 8.371, P < 0.001] in the experimental group were significantly different, while the TNF-α [(94 ± 22) ng/L vs. (107 ± 20) ng/L, t = 1.363, P = 0.188] expression showed no statistically significant difference. In the sham operation group, the colonic mucosa was intact and the glands were regularly arranged with little or no inflammatory cell infiltration. However, mice in the control group appeared derangement, deformation and lack of colonic mucosal epithelial glands, fuzzy connected structures of enterocytes, and extensive infiltration of inflammatory cells some of which had crypt abscess. The colonic epithelia of mice in the experimental group were basically complete without erosion and loss, glands were normally arranged, and the infiltration of inflammatory cells decreased more as compared to the control group. The results of immunohistochemistry showed that mice in the sham operation group had complete acinar structures of colonic epithelial cells and more Occludin proteins. The acinar structures of colonic epithelial cells in the control group were destructed and disappeared, and there were infiltration of inflammatory cells and less Occludin proteins. The acinar structures of colonic epithelial cells in the experimental group were complete with widened interacinar gaps, and the expression of Occludin protein increased as compared to the control group. Conclusion Probiotics can inhibit the reduction of Occludin of intestinal epithelial cells and stabilize barrier structures of intestinal mucosas, thus effectively improving the survival of septic mice. Key words: Probiotics; Sepsis; Occludin

This randomized-controlled trial demonstrates that an exercise consultation, based on the transtheoretic model of exercise behavior change, significantly improves short-term adherence to exercise.

The instructional-educational process in learning to swim plays a crucial role in children's development. Approaching methods and use in training practices of all ages and performance levels have been the subject of ever deeper research, so that the effectiveness of training in this area is increasingly visible. The issue of motivation in sports is a topical issue because it is essential for athletes' involvement and performance. Although material stimulation can be considered an important factor in motivating athletes, it is not necessarily enough to commit them to an extremely difficult and risky path, such as that of performance sports. Athletes' motivation stems from a complex combination of factors, including passion for sport, the desire to demonstrate their skills and talents, the aspiration to achieve personal and collective goals, and the desire to achieve recognition and satisfaction through sporting success. The purpose of the research is to identify the level of motivation for swimming and performance of children aged 9-10 years. Research method: the test Motivation for swimming of children aged 9-10 years (adaptation after N. Luskanova) The test was applied by the researcher in October 2022 to a number of 30 children aged 9-10 who practice swimming in the Tirgoviște Swimming Complex and CSȘ Tirgoviște Swimming Pool. Depending on age, we have, within the experimental group, 7 children aged 9 and 8 children aged 10. Within the control group, we identify 6 children aged 9 years and 9 children aged 10 years. We observe that the two groups are homogeneous, referring to the age variable. Regarding the gender of the subjects who are part of the group subjected to the experiment, we observe homogeneity in terms of gender, in each group there are 5 girls and 10 boys. The distribution according to the residential environment from which they come is as follows: from the experimental group 3 subjects come from the rural environment and 12 from the urban environment, and in the control group 1 subject comes from the rural environment and 14 from the urban environment The test results can be seen in Table 1. The difference between the values obtained between the experimental and control groups is not significant from a statistical point of view, since the values of the statistical indicators are close, we observe small differences between them.Table 1. Distribution of results on the Motivation for swimming test of children aged 9-10 years (adaptation according to N. Luskanova), experimental group and control group (n=20 boys)tabelThe differences between the two groups in the statistical indicators are variable, the difference between the averages is small, 0.6 points, in the standard deviation the difference between the groups is 0.77 points, the minimums recorded are equal to 15 points, and the difference between the maximums of the groups is 1 point. These results show homogeneity regarding the level of motivation between the experimental group and that of the control group. Table 2. Analysis of the level of motivation for swimming in the experimental and control groups, boystabelWe find that the level of motivation for swimming of the interviewed subjects is good. On the first level are ranked 2 subjects from the experimental group SC with 26 points and CR with 25 points, and from the control group subject MR with 25 points. These subjects have a very good motivation to practice swimming. On the second level, a number of 3 subjects from experimental group II are ranked with 24 points, AN with 23 points, AT with 20 points and six control subjects BM with 24 points, NA and RN with 23 points each, CI, AI and VE with 21 points each. The motivation of these subjects is characterized as good. Level III, respectively of relatively good motivation, has 5 subjects from the experimental group in the ranking: DF with 19 points, MG with 18 points, BL and IA with 17 points, GI with 15 points. In the control group, three subjects were registered on this level: BG with 19 points, RO with 18 points and DC with 15 points. None of the subjects was ranked on level IV-weak motivation and on level 5-very weak motivation. Overall, the motivation of 9-10 year olds for swimming is good and relatively good. In the following we will analyze the results recorded by the girls' teams. Table 3. Distribution of results on the Motivation for swimming test of children aged 9-10 years (adaptation according to N. Luskanova), experimental group and control group, (n= 5 girls)tabelThe difference between the values obtained between the experimental and control groups is not statistically significant as the values of the statistical indicators are close in value. The difference between the averages of the two groups is 1.04 points, the standard deviation being 2.82 points in the case of the experimental group and 1.49 in the case of the control group. The difference between the minimum values is 1 point, and between the maximum values is 4 points in favor of the experimental group. The results obtained by the girls from the two groups subjected to the experiment, in the Motivation for swimming test of children aged 9-10 years, are as follows: on the first level, with a very good motivation, only GM ranked with 26 points, from the experimental group , on level II, with good motivation, the subjects AF with 22 points and BM with 20 points from the experimental group and DV with 22 points, AN and IF with 20 points each from the control group are highlighted. On level III, relatively good motivation, there are EL with 19 points and MT with 18 points from the experimental group and BP and NO with 18 points each from the control group. Table 4 Analysis of the level of motivation for swimming in the experimental and control groups, girlstabelConclusions The analysis of the obtained data shows us that both groups subjected to the experiment, both in the case of boys and girls, have a good motivation for swimming, but there is a need to increase the level of motivation to maintain interest in this sport and to register performance, through specific activities. We believe that an increased level of motivation among children between the ages of 9 and 10 in swimming practice can have a significant impact on their development both in sports and personally. In correlation with the research results, we propose the following recommendations: - For each group of children, by age level, a psychological training program targeting the motivational component will be developed, which will be integrated into the training process. This program will be adapted to the specific needs of the group and will aim to adapt motivation and, implicitly, sports performance. - The psychological training program will include strategies and techniques to support the development of children's motivation for swimming, promoting commitment, the desire to excel and perseverance in training and competitions. This could include, among other things, visualization sessions, stability of personal goals, working with thoughts and emotions to maintain a positive mindset and focus on success.

It has been reported that during in vitro embryo culture reactive oxygen species (ROS) are generated and are detrimental to embryo development. A recent study (Smorag et al., Proc. 9th ICPR, 2013, 110) demonstrated that an addition of 1 mg mL–1 of hyaluronan (HA) to porcine embryo culture medium improves the development of zygote to blastocyst stage and the quality of produced embryos. Moreover, the embryos cultured with HA showed lower inner mitochondrial membrane potential (Romek et al., 2015 Proc. Symp. Progress in Cell Biology: Mitochondria and Chloroplast, Krakow, 31). Based on the beneficial effect provided by supplementation of HA during embryo culture, we investigated the ROS level in porcine embryos cultured with HA. Porcine zygotes were obtained surgically after flushing the oviducts of superovulated and inseminated gilts. In the experimental group, zygotes were cultured up to the blastocyst stage in NCSU-23 medium supplemented with 1 mg mL–1 of HA (CROMA, Pharma GmbH, Leobendorf, Austria), in an atmosphere containing 5% CO2 in air, at 39°C. In the control group, HA supplementation was omitted. To measure ROS level, embryos at the stages 2–4 and 8–16 cell, morula, and blastocyst (experimental group) and zygote, 2–4 and 8–16 cell, morula, and blastocyst (control group) were labelled with 5 μM CM-H2DCFDA dye (Molecular Probes Inc., OR, USA) for 30 min at 39°C. Labelled embryos were then examined under a Nikon Eclipse microscope with a CCD camera. The total amount of fluorescence emitted from each individual embryos and proportional to the ROS level was measured in arbitrary units. The data were analysed using one-way ANOVA and post-hoc Tukey test. ROS level (mean ± standard error of the mean) in the experimental group was 8.21 ± 2.65 (n = 25), 10.31 ± 3.13 (n = 18), 9.08 ± 2.89 (n = 21), and 20.45 ± 2.38 (n = 31) for 2–4 cell, 8–16 cell, morula, and blastocyst, respectively, whereas in the control group was 9.15 ± 3.43 (n = 15), 7.11 ± 3.13 (n = 18), 8.67 ± 3.04 (n = 19), 11.47 ± 2.46 (n = 29), and 54.74 ± 2.89 (n = 21) for zygote, 2–4 cell, 8–16 cell, morula, and blastocyst, respectively. For experimental and control groups, ROS levels remained unchanged up to morula. On the contrary, at the blastocyst stage from the experimental group ROS level decreased significantly (P ≤ 0.05) in comparison with blastocysts from the control group. In conclusion, porcine blastocysts derived from zygotes cultured with supplementation of 1 mg of HA possess a significantly lower ROS level than blastocysts cultured without HA. This suggests that HA supplementation in culture medium can reduce the ROS level in porcine cultured blastocysts. The project was funded by the National Science Center based on decision number DEC-2012/07/B/NZ9/01326.

Statement of the problem: A research titled “A Comparative Study to Assess the Effectiveness of Helfer’s Skin Tap Technique Versus Routine Technique on Pain Reduction among Patients Receiving Intramuscular Injections in Selected Hospitals in Greater Noida.Aim: The aim of study is to assess the effectiveness of Helfer’s Skin Tap Technique Versus Routine Technique on Pain Reduction among Patients Receiving Intramuscular Injections in Selected Hospitals in Greater Noida.Objectives of the study · To assess the level of pain experienced by the patients receiving intra muscular injection using routine technique. · To find out an association between levels of pain experienced with selected demographic variables.Methodology: A qualitative research approach was used and the research design adopted for the present study was Experimental research design. The target population for the study was patients who all are receiving IM injection in selected hospitals, greater Noida. Convenient samplingTechnique was used during study. Simple size 60 data analysis was done based on objectives and hypothesis of study.Results: In this research the findings show that maximum number of patients under experimental group selected for the study who satisfied the inclusion criteria were N=12 (40%) where in control group N=13(43.4) were among 40-49 years, followed by N=8(26.7%) in both experimental and control group, were from 30-39 years further N=8(26.7%) in experimental and in control N=7(23.3) were of 20-29 years and N=2(6.7%)in both experimental and control group were of 50-60 years. In this research the researcher has selected N=60 (100%) as adult males both in experimental and control group. The majority of the patient N=22 (73.3%) experimental group N=28(93.3) control group were married, whereas N=8(26.7%) experimental group N= 2(6.2) control group were unmarried. Widower and divorced were N=0 in both experimental and group.The most of the patients lived in rural areas where as N=26 (86.7) experimental group and N=25(83.3) control group, followed by patient lived in urban areas where as N=4(13.3) experimental group N=5 (16.7) control group. Majority were N=11 (36.7%) in experimental group & N=16(53.3%) in the control groups subjects were can read and write & the experimental group N=10 (33.3%) and N=8 (26.7%) of subjects in the control group have no formal education. Whereas N=7 (23.3%) of the subjects in the experimental group and N=5 (16.6%) of the subjects in the control group have middle education. And N=2 (6.7%) experimental group N=1 (3.3%) in control group. Most of the patient were working in experimental group N=16(53.3) same in control group N=19(63.3) whereas N=14(46.7) experimental group N=11(36.6) control were not working. The level of pain perception during IM injection in Experimental group. Majority of the samples (100%) perceived minor pain with Helfer’s skin tap. whereas majority (53.3%) moderate and (46.7%) had minor pain with routine technique.Conclusion: The present study revealed that there was a significant reduction in pain among patients after administration of Helfer's skin tap technique. Thus it proved to be an effective treatment for pain.

Introduction Pregnancy and birthing are two supernatural processes that form the creation of life. Many pregnant women prefer caesarean section due to labour pain. To reduce the complications and enhance the early recovery of mother, early ambulation is very effective. Caesarean section mothers still lie straight in bed for minimum 24 hours. Through the research study, I would like to encourage the post-partum mothers for early ambulation and check the effects of early ambulation on involution of uterus. Objective: To assess the involution of uterus in control and interventional group among post-natal caesarean mothers in pre-test. To evaluate the effectiveness of selected intervention in experimental group on involution of uterus after early ambulation. To find the association between pre-test intervention score with selected socio demographic variables. Method: The study design was quasi experimental research design in which total 70 samples were selected through purposive sampling technique, 35 samples were in control group and 35 samples were in experimental group. In this study, researcher assessed fundal height just after 6 hours of caesarean section delivery and initiated early ambulation which involves Assist patient in sitting position, assist in standing position and walking, breathing exercises, kegal exercises and leg sliding exercises. fundal height is monitored in experimental group after intervention and in control group also, to measure the differences between the fundal height. Results: The findings showed that the pretest mean score of control group was 17.23 with SD 0.899 similarily in experimental group was 17.10 with SD 0.778. The post-test mean score for control group was12.986 with SD 0.660 similarly in experimental group 10.083 with SD 1.376. P value in pretest was 0.5338 which was no significant showed that no significance difference between experimental and control group before intervention and p value of post test in control and experimental group is 0.0001 which was significant showed that there was significant difference in post test level of uterine involution among post natal caesarean mothers in control and experimental group. The mean difference between pretest &amp; posttest of control group was 4.249 and in experimental group means difference was 7.026 this showed that there was significant effect of early ambulation in involution of uterus among post natal caesarean mothers. There was significant mean difference between post test of control and experimental group showed significant reduction in fundal height after early ambulation in experimental group. Conclusion: Therefore early ambulation was effective in reducing fundal height after caesarean section in post natal mothers.

A study of two 100-person groups of juvenile delinquents born in 1959 was conducted in the years 1981‒1985 at the Department of Criminology, Institute of Legal Studies, Polish Academy of Sciences. The first (experimental) group consisted of boys randomly selected from the total of 225 juveniles born in 1959 who had committed at least on offence while intoxicated. The other (control) group were 100 randomly juveniles selected from the entire population of 8196 juvenile delinquents born in 1959. None of the juveniles selected for the experimental group happened to find themselves in the control group as well. As shown by the findings, the juveniles who had committed at least one offence while intoxicated were much more demoralized as a group than the whole of juvenile delinquents. It seemed interesting, therefore, to follow the further fates of both groups as adults. The follow-up period was 7 years; until that time, all of the examined persons reached the age of 25 when the average Polish man be- comes stabilized to some extent, having graduated from university, worked for several or a dozen years (upon completion of secondary or elementary education respectively), and frequently having also established a family. Data on the life situation of the young men from both examined groups on their 25th birthday were obtained from the following four sources: ‒ the Central Register of Convicted Persons kept by the Ministry of Justice, and the Register of Convicted and Detained Persons where criminal records of the entire sample were checked; ‒ files of criminal cases of all men with criminal records (47.0% of the experimental and 35.0% of the control group); the files concerned criminal proceedings before common courts for offences committed after coming of age; ‒ questionnaire survey of 63.0% of the experimental and 66.0% of the control group; ‒ inquiry submitted to the sobering-up stations concerning the entire sample. As shown by the findings, 24.0% of the experimental and 13.0% of the control group established their own families before the age of 25. The proportions are high, as regards the experimental group in particular: erly in the 1980s, the newly married constituted about 10% of the total male population aged 20‒24 in Poland. The mean educational level was higher in the control compared to the experimental group; this concerns first and foremost cases of education higher than the elmementary technical (of which there were two in the experimental compared to ten in the control group). Moreover, no cases of illiteracy could be found in the control group, compared to one such case in the experimental group. Of all the men of the experimental group concerning whom data could be obtained, 80.4% had a regular job, and 19.6% stayed out of job or worked casually. Of the control group, 80.0% had a regular job (33% combining job with school), 18.3% stayed out of job or worked casually, and one person had entered university. The number of convicted persons in the experimental group (47) was larger compared to the control group (35) by 12.0%, the difference being significant. Also relapse into crime was higher in the expenmental group (l5 vs. 11 cases). The first offence committed by those convicted as young adults was mainly one against property: 35 cases in the experimental group (66.0% of all those convicted) and 28 cases in the control group (80.0%). The second most frequent offence of members of the experimental group was an aggressive act: against life and health, freedom, personal dignity and inviolability (10 persons, i.e. 18.8% of all those convicted). The offences of this type included: bodily injury (Art. 156 of the penal code – 3 persons, i.e. 6.4%); participation in a brawl or beating (Art. 158 and 159 – 1 person, i.e. 2.1%); infringement of bodily inviolability (Art. 182 – 3 persons, i.e. 6.4%); assault against a public functionary (Art. 233 and 234 – 1 person, i.e. 2.1%); insult against a public functionary (Art. 236 – 1 person, i.e. 2.1%). In the control group, 6 cases of such offences could be found (9.1% of all those convicted); yet the only offence under Art. 148 1 of the penal code, that is homicide, had been committed by a member of that goup.The other discussed figures and proportions were respectively: Art. l58, 159 – 3 persons, i.e. 8.6%; Art. 182 – 1 person (2.9%); Art. 233, 234 – 1 person (2.9%); and Art. 236 – 1 person (29%). Beside offences, the two groups manifested also other synptoms of social maladjustment. The symptoms found most often in both groups were: “contacts with persons known to the police as delinquent” and stays at the sobering-up station. As regards the experimental group, the third frequent symptom were brawls in the place of residence followed by bad opinion with neighbors, hooliganism, and avoidance of work. In the control group, avoidance of work ranked third, followed by bad opinion with neighbors, hooliganism, and brawls at the place of residence. This ranking of frequency of the symptoms of social maladjustment points to a greater aggressiveness of the young men from the experimental group. On the 63 young men from the experimental group concerning whom data could be obtained, 62 (98.0%) drank alcohol. In the control group 59 (89.0%) of the 66 concerning whom data could be obtained were drinkers. The group of drinkers included all those who had drunk several times a week already as juveniles, and 70% of those who had drunk once a week. In the control group, drinkers included 91.7% of those who had drunk as juvoniles (11 of 12 cases). Of those who had drunk as juveniles in the control group, 66.7% (8 cases) were convicted as adults. As shown by the discussed data, young men from the experimental group – those who committed as juveniles at least one offence while intoxicated prove much inferior in terms of the social situation to other men who also committed offences as juveniles but did not drink alcohol. Therefore, early alcohol consumption among juvenile delinquents is an important factor of a negative prognosis as to the further fates of such persons. A number of postulates have been formulated, addressed at the prosecuting agencies, criminal justice, and institutions designed to assist persons in extraordinary situation. With respect to the present sample, all such postulates acquire special importance and must be met without fail. Niniejszy artykuł stanowi kontynuację opracowania: Nieletni sprawcy przestępstw popełnionych pod wpływem alkoholu, „Archiwum Kryminologii” 1991, t. XVIII.

This study was designed to assess the impact of problem based learning on students’ critical thinking in science at elementary level. Major objectives of the study were to examine critical thinking’s of the students, to practice problem based learning through activities, and to evaluate critical thinking of the students after problem based activities. The study population was encompassed all students of 8th grade (20172) within District Muzaffar Garh. Sample consisted of total 50 students from within the same district. Twenty five students out of 40 were selected from Government Boys Elementary School Arshad Abad by using simple random technique as experimental group. Twenty five students out of 27 were selected from Government Boys Elementary Khara by using simple random technique as control group. The designs of study were pretest-posttest and Quasi experimental in nature. Problem based learning treatment pattern was applied. During 16-week treatment duration, the experimental group and the control group were treated with problem-based learning and taught through traditional lecture-based methods respectively. Data was collected from the control and experiment groups before and after four month problem-based learning through pre-test and posttest. After data collection, analysis of the data collected done through descriptive statistics by using mean, median, mode and standard deviation (SD) and inferential statistics by using t-test. Paired sample t-test was applied to measure significance difference value between the means of experimental and control group. T-test value is greater between experimental group and control groups in pretest than the significance value that shows there is significance statistical difference between experimental group and control group performances in pretest. Performance of experimental group is better. Value of T-test is greater between experimental and control groups in posttest than the significance value shows there is significance statistical difference in experimental and control groups performances in posttest. Experimental group performance is better. The experimental group performed better as compared to performance of control group in both pretest and posttest. But the difference in means of pretest of experimental and control group is very small than difference in means of posttest of experimental group and control groups. It depicts through problem based learning students’ critical thinking is highly improved.

We aim to use the ovariectomized cynomolgus monkey to observe the effect of long-term changes of sex hormone levels on ocular surface. Six healthy adolescent cynomolgus monkeys were randomly assigned to control and experimental groups (n=3/group). Bilateral ovariectomy was performed in experimental group, and sham operation was performed on control animals with their ovaries reserved. The concentrations of serum estradiol and testosterone in cynomolgus monkeys' lower-limb venous were detected by radioimmunoassay before surgery, and at 6, 12, 18, and 24 months after the surgery. Phenol red cotton thread test, tear osmolarity, corneal fluorescein staining, and conjunctival impression cytology were performed to evaluate the ocular surface conditions before surgery, and at 6, 12, 18, and 24 months after the surgery. The histological examinations of the bulbar conjunctiva and labial glands tissues were performed at 12 and 24 months after the surgery. There were no significant differences in the serum level of estradiol and testosterone between the experimental and control groups before surgery, at 6, and 12 months after surgery (P>0.05). Serum level of estradiol and testosterone were significantly less in experimental versus control group at 18 and 24 months after surgery (P<0.05). There was no significant differences in phenol red cotton between the experimental and control groups before surgery, at 6, 12, 18, and 24 months after surgery (P>0.05). Tear osmolarity had no significant differences between experimental and control group before surgery (P>0.05), and it was significantly higher in experimental versus control group at all four time-points after surgery (P<0.05). The number of conjunctival goblet cells had no significant differences between experimental and control group before surgery, at 6 months after surgery (P>0.05), and it was significantly lower in experimental versus control group at 12, 18, and 24 months after surgery (P<0.05). There were no significant differences in the corneal fluorescein staining score between the experimental and control groups before surgery, at 6, 12, and 18 months after surgery (P>0.05), and it was higher in experimental than control group at 24 months after surgery (P<0.05). In addition, inflammatory cell infiltration in bulbar conjunctiva and labial glands tissues was observed in experimental group and was unobserved in control group at 24 months after surgery. Ovariectomy led to decreased sex hormone levels in cynomolgus monkey. In turn, tear osmolarity was increased, the number of conjunctival goblet cells were decreased, the corneal fluorescence staining was increased, and the pathological examination of the bulbar conjunctiva and labial glands tissues were abnormal, phenol red cotton thread test did not change significantly.

Aim of the study: The study aim to find the effectiveness of kriya yoga to reduce the anxiety level among COVID nursing staff working at selected hospital. Objectives of the study: 1. To assess the pre test level of anxiety among the staff nurses of COVID unit at selected hospitals among experimental and control group.2. To assess the effect of kriya yoga on level of anxiety by comparing the pre test and post test level of anxiety among experimental group.3. To assess the effect of kriya yoga on level of anxiety by comparing the post test level of anxiety among experimental and control group.4. To determine the association between pre test level of anxiety with their selected demographic variables among experimental and control group. Method: True experimental research design and A quantitative research approach was carried out on 60 nursing staff selected by simple random sampling technique to test the effectiveness of kriya yoga. The data was collected by using Beck anxiety inventory scale consist 21 questions. Results: Age distribution of staff nurses in the experimentalgroup: 6.67% aged 24 -26 years 10% aged 27 -29 years 6.67% aged 30 -32 years 76.67% above 33 years Age distribution of staff nurses in the control group: 3.33% aged 24 -26 years 13.33% aged 27 -29 years 10% aged 30 -32 years 73.33% above 33 years Gender distribution of staff nurses: Experimental group: 30% males, 70% females Control group: 20% males, 80% females Residential area distribution of staff nurses: Experimental group: 53.33% rural, 46.67% urban Control group: 46.67% rural, 53.33% urbanMarital status of staff nurses: Experimental group: 76.67% married, 23.33% unmarried Control group: 86.67% married, 13.33% unmarried Impact of COVID -related deaths in family: Experimental group: 86.67% reported zero deaths, 10% reported one death, 3.33% reported more than two deaths Control group: 86.67% reported zero deaths, 10% reported one death, 3.33% reported two deaths Duration of work in the COVID unit: Experimental group: 3.33% worked for 3 months, 3.33% for 6 months, 60% for 1 year, and 33.33% for more than 1 year Control group: 3.33% worked for 6 months, 80% for 1 year, and 16.67% for more than 1 year Family income per month: Experimental group: 53.33% reported 20000 Rs, 46.67% reported more than 20000 Rs Control group: 60% reported 20000 Rs, 40% reported more than 20000 Rs Type of family: Experimental group: 63.33% from nuclear families, 26.67% from joint families, 3.33% from single parent families, and 6.67% from extended families Control group: 53.33% from nuclear families, 33.33% from joint families, 13.33% from single parent families, and no one from extended families History of COVID infection: Experimental group: 40% reported one time infection, 3.33% reported two times, and 56.67% reported no history of infection Control group: 33.33% reported one time infection, 3.33% reported two times, and 63. 33% reported no history of infection. Conclusion: The following conclusion were drawn from the findings of the present study. Their chapter shows that kriya yoga therapy is a simple non pharmacological intervention which should be carried out independently in the field of nursing. The overall experience of conducting this study was enriching hence it gives an opportunity to 185 the investigator to acquire new information as well as learning experience.The experience of the investigator during the study and the findings helped the investigators to give suggestion and the recommendation for further studies.

Introduction: Aldehyde dehydrogenases (ALDH), particularly ALDH1B1 has been shown to have increased expression in both human and APCMin mice colorectal tumors. Studies have proposed the use of ALDH1B1 as a potential novel human colorectal tumor marker. Previous in vitro studies have shown that ALDH1B1 plays a critical role in colon carcinogenesis. The goal of this study is to understand the in vivo role of ALDH1B1 in colon carcinogenesis using a newly developed mouse model, APCMinAldh1b1−/− mice, through characterization of its phenotype in tumor growth and development, and elucidating the underlying potential molecular mechanisms. Methods: We generated APCMinAldh1b1−/-. At 12-14 weeks old, the experimental group APCMinAldh1b1−/- (n=6) and control group APCMinAldh1b1+/+ (n=7) were euthanized. Tumor counts, size and burden for each intestinal segments were recorded. H&amp;E staining was performed and observed for tumor types and counts. Immunohistochemistry using the markers Ki-67 (proliferation), Cleave Caspase 3 (apoptosis), KRT20 (differentiation) were done in colon. IHC of retinoic acid binding proteins (anti-CRBP2 and anti-FABP5) were also performed Results: The experimental group had shown significantly higher total average intestinal tumor burden (6.8 fold) (p=0.0007) and total average tumor counts observed (3.6 fold) (p=0.02). In the colon, there was a 3.5-fold increase in average tumor burden (p=0.017) with a 2.7-fold increase in average tumor size (p=0.025)in the experimental group. We also noticed a trend of increased average tumor counts observed in the colon of the experimental group although statistically insignificant. Average tumor counts of ileum observed was 9-fold higher in experimental group with only 1 mice from control group showed observable small tumor growth which did not meet criteria for tumor size and tumor burden calculation. There were no significant differences in observable tumor counts, tumor size and tumor burden in jejunum. Histologically, all tumors were pedunculated adenocarcinomas. Many unobservable small tumors less than 1mm width and length were seen in the H&amp;E of both ileum and jejunum in the experimental and control group. In the control group, there was less observable tumors compared to the experimental group although the tumor counts on H&amp;E in both groups showed no major differences. This indicates that the tumor size in the experimental group was larger than the control group. On the contrary, average tumor counts of colon in H&amp;E were lower compared to tumor counts observed in both control and experimental groups, indicating low number of small unobservable tumors in colon. Although the tumor counts of colon on H&amp;E and observation between the two groups were statistically insignificant, we noticed a pattern of increased tumor counts in the experimental group suggesting a potential increase in tumor incidence. Immunohistochemical staining of colon is ongoing. Conclusion: ALDH1B1 reduced tumor growth rate in the ileum, and reduced tumor size in the colon suggesting its role in tumor promotion and progression. A larger number of experimental and control group is required to establish its potentially tumor-initiating role in the colon. Further characterization of ALDH1B1 in colon carcinogenesis is ongoing. We are also elucidating the role and regulation of retinoic acid signaling by ALDH1B1 in colorectal tumor growth. Citation Format: Wan Ying Tan, David. J. Orlicky, Ying Chen, Yatrik M. Shah, David C. Thompson, Vasilis Vasiliou. Role of aldehyde dehydrogenase 1B1 in colon carcinogenesis in a newly developed mouse model [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 6141.