Abstract
1. 1. Rainbow trout held in net pens for 4 weeks in three locations contaminated by heavy metals developed elevated levels of hepatic metallothionein but no acclimation to a combination of zinc, copper and cadmium. 2. 2. Toxicity of the mixture of zinc, copper and cadmium in a ratio of 400:20:1 increased with temperature in the range of 6–21°C. 3. 3. Hepatic metallothionein concentrations after 4 weeks of exposure in situ was correlated with the degree of contamination as measured by zinc concentrations ( P < 0.01).
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