Abstract
Trypanosoma evansi is definitely one of the most common pathogenic trypanosomes in all over the world, obviously infecting domestic and wild animals. Before it contributes to even more economic losses, a fast and confirmatory early diagnosis is a must to curb the spread and development of the disease. The current study was aimed to identify Trypanosoma spp. in buffalo blood by blood smear analysis and polymerase chain reaction (PCR) as well as comparison of these two methods. This investigation was performed on 475 buffaloes with anaemia, recurrent fever, muscular weakness and loss of appetite. Out of 475 blood samples, 57 were found anaemic (Hb
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