Helicobacter pylori Infection in Children: A New Focus

Abstract

Helicobacter pylori Infection in Children: A New Focus Background: Helicobacter pylori (H. pylori) infection places a heavy burden on medical and economic resources in worldwide. Standard diagnosis requires the presence of established H. pylori gastric disease. Thus, rapid and convenient identification and treatment of children at risk for developing infection is not possible. This slides show the evidence of H. pylori antigen in the mouth can be used to identify people at risk for disease, Special for children. Family Aggregation: The aspect of children are salient features and familial aggregation that are particularity of H. pylori infection of childhood. In children infected with H. pylori, the main source of infection is connected with family members and caregivers, especially in close contact with the mother and children, through the mouth - mouth, dung - oral transmission. H. Pylori infection rate of Children: Children are most susceptible to infection by H. pylori. According epidemiologic Meta analysis , the rate of infection estimate approximately 39.55% in children aged 1~5 group, but 43.90% at same age group in high prevention of stomach cancer territory. Symptomatic aspects: H. pylori infection usually associated with indigestion, chronic diarrhea, frequent recurrent abdominal pain as well as many extragastric disease, such as deficiency anemia, irondeficiency anemia, slow developing, chronic Urtica Cannabina Oral H. pylori infection: Oral infection of H. pylori associated with periodontitis, caries and ozostomia. Oral cavity is second location of H. pylori infection that is major source of stomach infection and key f Diagnosis of H. pylori in Children Endoscopy examination to diagnosis is not suitable for children. BUT C13 is most common technology use for diagnosis in children, however limited the capacity of age below 6, there are false negative results may occur. Principle of H. pylori Saliva test (HPS): H. pylori urease antigen was specifically detected in saliva using a lateral flow, immuno-chromatographic test. The principle of this test is similar to UBT C13 in detecting urease released by H. pylori. The test employed monoclonal antibodies which were developed against semi-purified urease protein. HPS is suitable methold for children. HPS specificity An important feature of H. pylori is that it can be divided into nontoxigenic and toxigenic strains that produce a vacuolating toxin (VacA). We use H. pylori, which carry virulence factor CagA (cytotoxinassociated gene A) and VacA that markers released pylori urease. Then we made antibodies from VacA and CagA. HPS can specifically detecting H. pylori carry VacA and CagA