Abstract
Human skin fibroblasts were attached to a plastic foil and cultivated in a batch calorimeter. The cultures exhibited a characteristic heat profile, whose different phases were attributed to the processes of spreading and growth of the seeded cells, to cell multiplication and to the metabolism of maintenance. An enthalpy change of (1.5 ± 0.3) μJ per dividing cell and, during confluency, a heat production of (40 ± 10) pW/cell were determined. The monolayer cultures could be kept alive and free of microbial contamination for more than 5 days within the calorimetric vessel.
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