Abstract
Objective of this study is to investigate the changes of gene expression in rat brain induced by 2,4 GHz wireless radiofrequency electromagnetic field (WiFi -RF EMF). Total RNA was extracted immediately and purified from the rat brain, after 12 hours/day exposed or sham-exposed to a frequency of 2,4 GHz WiFi –RF- EMF for 14 days. Roche-Nimblegene-Agilent Microarray System was applied to investigate the changes of gene expression in rat brain. Roche-Nimblegene-Agilent Genespring Software for Microarray Analysis was used in bioenformatic analysis. After 14 days of WiFi -RF EMF exposure, 69 genes in experimental group showed statistical significant down regulation compared with the control group. Ten genes in experimental group showed statistical significant up regulation compared with the control group. In this study, it was detected that WiFi -RF EMF exposure to brain cells resulted with the down and up regulation of transcription level of some important genes.
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