Abstract
Oral squamous cell carcinoma is a common malignant lesion. ROCKs proteins are associated with pathogenesis and progression of human tumours. This study aimed to evaluate the functional role of ROCKs in the regulation of cell proliferation of oral squamous cell carcinoma in vitro. BrdU incorporation assays and KI-67 immunoexpression were performed by using SCC-4 cell line from oral squamous cell carcinoma. Control and treated cells: HA-1077 (25, 50 and 100 μmol/l), 50 μmol HA-1077 and Y-27632 30 μmol/l, Y-27632 30 μmol/l were cutured for 6 h. The number of SCC-4 cells treated with: HA-1077 (25, 50 and 100 μmol/l), HA-1077 50 μmol/l and/or Y-27632 30 μmol/l was significantly lower than control cells in BrdU assay [F (5.17) = 443.818, p<0.0001] and in KI-67 assay [F = 192.595, d.f. = 5,17; p<0.0001]. The results obtained suggest that the pathways that evolve ROCKs proteins play an important functional role in the positive regulation of cell proliferation in oral squamous cell carcinoma.
Highlights
This study aimed to evaluate the functional role of ROCKs in regulation of cell proliferation in SCC-4 cells from Oral squamous cell carcinoma (OSCC) in vitro, using its specific inhibitors HA-1077 and/or Y-27632
It was significantly lower than the control, the number of proliferating cells treated with HA-1077 50 μmol/l associated with Y-27632 30 μmol/l (16.81±0.98 cells, p
This study demonstrated that ROCKs proteins play an important functional role on the proliferation of SCC-4 cells obtained from OSCC
Summary
Despite the improvement in the diagnosis of head and neck cancer and the large number of research and advances in oncology, morbidity and mortality rates remain high, representing the sixth most common cancer worldwide (Massano et al, 2006). Oral squamous cell carcinoma (OSCC) accounts for 90% of cases (Travassos et al, 2017) and the risk factors associated with its development are: smoking, alcoholism and HPV infection (Ragin et al, 2016). The association between alcohol and tobacco due to its synergistic effect multiplies the chances of developing the disease (Lin et al, 2016). It is necessary to identify proteins that regulate the proliferation of OSCC cells and enable the development of a specific therapy capable of effectively blocking this signal transduction pathway (Wei et al, 2016). The occurrence of metastases is common and aggravates the prognosis of the disease, being the main cause of the deaths (Stucky et al, 2017)
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