Abstract
To prevent the introduction of highly pathogenic avian influenza (HPAI) into Japan, we examined chicken imported from China for viruses. For virus isolation, we used embryonated chicken eggs and a total of 473 samples of frozen meat and bone marrow collected between June 2001 and July 2002. The Newcastle disease virus (NDV) was isolated from 11 (2.3%) specimens and the avian influenza virus (AIV) from 13 (2.7%) specimens. No HPAI virus was included. On the basis of pathogenicity-index tests, all of the NDV isolates were identified as velogenic strains with112RRQKR-F117sequences at the F-protein cleavage site, as is typical of virulent NDV strains. As a result of phylogenetic analysis basedon the F gene, all NDV isolates were classified as belonging to genotype VII, which is prevalent mainly in East Asia and is especially closely related to strains prevalent in China. All AIV isolates were identified as H9N2 subtypes. Intravenous inoculation tests using 3AIV isolates showed no disease symptoms in chicken. Phylogenetic analysis based on the HA gene showed all AIV isolates to belong in the cluster of isolates found mainly in chicken from China.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Japan Veterinary Medical Association
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
