Growth response of Oryza sativa seedlings to graphene oxide and its variability among genotypes

Abstract

With the extensive utilization of graphene nanomaterials, they inevitably enter our environment. The potential phytotoxicity and environmental impact of graphene oxide (GO) have recently attracted much attention. We designed the experiment based on seed germination, seedling morphology, physio-biochemical properties, and antioxidant enzyme activities of five rice genotypes (9311, MH63, R527, K866, and Nipponbare) under six concentrations of GO (0, 5, 10, 50, 100, and 150 mg dm-3). We studied the effects of different concentrations of GO on germination index (GI), shoot length (SL) and root length (RL), adventitious root number, shoot and root fresh masses, root/shoot ratio, chlorophyll (Chl) content, malondialdehyde content, and activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). Graphene oxide treatments significantly enhanced seed germination and root growth and inhibited shoot growth of all genotypes. Furthermore, we found a significant genotype-dependent response to GO treatments. According to the relative increment trend of GI, SL, and RL, root/shoot ratio, antioxidant enzyme activities (CAT, POD, and SOD), and Chl content, 'R527' showed more tolerance to GO treatments than the other four genotypes. The 'MH63' and 'K866' were more sensitive than 'Nipponbare' and '9311'. It indicates that the GO-tolerant genotype might avoid free radicals damage from GO by increased antioxidant enzyme activities. Moreover, we should consider the genotype differences when evaluating the potential phytotoxicity of GO and environmental risk to ecosystems.