Growth performance and gut function of piglets weaned at four weeks of age and fed protease-treated soya-bean meal

Abstract

Two experiments were carried out to investigate the effects of the protease-treatment of soya-bean meal (SBM) on its composition and nutritive value when fed to piglets weaned at 4 weeks of age in cereal-based diets. In experiment 1, two proteases, active at either alkaline (protease P1) or acid pH (protease P2), were compared with alkali or acid treatment alone. In experiment 2, treatment with protease P2 at acid pH was compared with untreated-SBM, acid-treated SBM, a soya protein concentrate or skim milk powder+fishmeal. Protease treatment increased SBM alpha amino nitrogen concentrations and reduced in vitro SBM antigenicity. Protease P1 reduced in vitro antigenicity to a greater extent than protease P2. However, protease P1 hydrolysed SBM polypeptides non-specifically whereas protease P2 preferentially hydrolysed a high molecular weight polypeptide. In experiment 1, piglets (12 diet −1) fed alkali-treated SBM gained more weight than piglets fed acid-treated SBM in the first 7 days after weaning. Protease P1 did not improve live-weight gain compared with alkali-treatment whereas protease P2 did improve live-weight gain compared with acid treatment. In experiment 2, piglets (24 diet −1) fed protease P2-treated SBM or skim milk powder+fishmeal gained significantly more weight over the first 7 days post-weaning than piglets fed untreated SBM. In neither experiment were there significant differences between diets in piglet serum anti-SBM specific antibodies. There were no consistent changes in small intestine enzyme specific activities in response to the diets fed. Histopathological examination of the small intestine of piglets in experiment 2 revealed no evidence of enteric disease. Neither were there any differences between diets in villus height or crypt depth.