Growth inhibition of cultured cancer cells by <i>Ribes nigrum</i> leaf extract

Abstract

<abstract> <p>The present article includes data on the possible selective cytotoxic effect of extract of <italic>Ribes nigrum</italic> L. growing at high Armenian landscape. For this purpose, different non-cancer (microglial BV-2 wild type (<italic>Wt</italic>), acyl-CoA oxidase 1 (ACOX1) deficient (<italic>Acox1<sup>−/−</sup></italic>) and cancer (human colon adenocarcinoma HT29 and human breast cancer MCF7) cell lines were applied. <italic>R. nigrum</italic> leaf ethanol extract showed a growth inhibition effect towards HT29 and MCF7 cells started from 6 h of treatment at the concentration of 0.5 mg/mL DW. The lowest concentration (0.125 mg/mL DW) of the investigated extract expressed cytotoxicity after 72 hours following cancer cell treatment. In contrast to the cancer cells, in the case of the tested non-cancer cells, cytotoxic effect was not observed at the applied concentrations. The extract sub-cytotoxic concentration, in this case, was reported to be the 1 mg/mL DW. Further investigations are needed to confirm the selective cytotoxicity and possible action mechanisms of the leaf extract of <italic>R. nigrum</italic>.</p> </abstract>