Abstract
Increasing the success rate of in vitro maturation (IVM) for human oocytes has a major clinical significance. Previous studies have shown that growth hormone (GH) added into IVM medium could promote IVM of oocytes from non-human beings. However, few studies on systematic IVM for human oocytes with GH have been reported. Human germinal vesicle (GV) oocytes collected for IVM were cultured with different concentrations of GH to optimize the concentration. Metaphase II (MII) stage oocytes obtained from IVM were fertilized by intracytoplasmic sperm injection (ICSI). Maturation rate, fertilization rate, and blastocyst rate were assessed after IVM with or without GH. Furthermore, gene expression profiles were compared in oocytes between the two groups using single-cell RNA-seq. The optimal concentration of GH for IVM was 200 ng/ml, and the maturation rate of this group reached 70% which was double that of the control group (35%, P = 0.004). The fertilization rate (73.1 vs. 60.3%) and blastocyst rate (25.0 vs. 15.5%) both had an increasing trend in the GH group compared to controls. Single-cell RNA-Seq and real-time PCR data showed that GH could significantly enhance the expression of genes associated with meiotic progression and embryo development, such as AURKA (aurora kinase A, P = 0.007), PDIA6 (protein disulfide isomerase family A member 6, P = 0.007), LINGO2 (leucine rich repeat and Ig domain containing 2, P = 0.007), and CENPJ (centromere protein J, P = 0.039). Taken together, GH could promote maturation of human oocytes, probably through accelerating meiotic progression, balancing redox homeostasis of cellular environment, and promoting oocyte developmental competence.
Highlights
Oocyte in vitro maturation (IVM) is a patient-friendly and less expensive human assisted reproduction technology (ART)
We found that growth hormone (GH) could promote human oocyte maturation in vitro, and the concentration was optimized
We compared transcriptome profiles of human oocytes matured in vitro with GH or not by single-cell RNA-seq, which suggested that GH probably work by accelerating meiotic progression, balancing redox homeostasis of cellular environment, and promoting the oocyte developmental competence
Summary
Oocyte in vitro maturation (IVM) is a patient-friendly and less expensive human assisted reproduction technology (ART). It can effectively decrease the risk of ovarian hyperstimulation syndrome (OHSS) due to its low dosage of gonadotropin administration, and can simplify treatment procedures by avoiding frequent ultrasonography. To examine the effects of GH on human oocyte IVM, the immature oocytes derived from ICSI cycles could be made use of to perform a preliminary study These oocytes are often discarded and considered to be useless. These immature oocytes have their own value They can achieve maturation and early embryo development in vitro [24], and obtain pregnancy [25]. Single-cell RNA-seq was employed to explore the mechanism of GH
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