Abstract
Ten culture media were tested for their effect on growth and sporulation of Cercospora cruenta and C. canescens isolated from field-infected cowpea (Vigna unguiculata). There was very little mycelial growth and little or no conidia production on most media. Oxoid agar supplemented with V-8juice supported best growth and conidial production. Flooding V-8 juice agar with a conidial suspension resulted in multiplicity of tiny colonies with resultant increased sporulation. Conidia produced in culture were slightly longer than those produced in lesions on field-infected leaves and the degree of septation varied with the type of culture media. The width of conidia showed no apparent variation regardless of the culture media. More active mycelial growth but less conidial production was observed at agar concentration of 3% than at lower concentrations. Optimum temperature for mycelial growth and conidial production was 24–25 °C. Growth and conidial production were inhibited in complete darkness but stimulated by light.
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