Abstract
This study aimed to isolate and identify the fungi associated with white and yellow corn grain and peanuts seeds samples collected from different distracts at Cairo, to use Morus nigra leaves extract for the green synthesis of AgNPs and to evaluate the antifungal potency and anti-aflatoxin production of the synthesized AgNPs. White and yellow corn and peanut seeds were collected from 4 different districts of Cairo, mycoflora were isolated on Potato dextrose agar (PDA), Nash and Coon’s media. Sixteen fungal species belonging to 7 genera were recovered and identified during this study. Uv/Vis absorbance at 425 nm and particles sizes ranged from 4 to 8 nm. Both AgNPs and the plant extract showed a strong antifungal activity against P. carryophylum; F. verticillioides; A. flavus; A. terreus and F. oxysporum using different techniques. Moreover, AgNPs was more effective than the plant extract against the tested fungi and anti-aflatoxin production by A. parasiticus. It could be concluded that the Morus nigra leaves extract can be used for the syntheses of AgNPs and also as antifungal of plant disease. Thus, Morus nigra and AgNPs could be used as effective, safe and ecofriendly antifungals to prevent fungal growth and subsequent aflatoxins production.
Highlights
Seed-borne fungi have been found to affect the growth and productivity of crop plants including legumes and cereals (Weber et al, 2001), in addition some species can produce health-damaging mycotoxins
This study aimed to isolate and identify the fungi associated with white and yellow corn grain and peanuts seeds samples collected from different distracts at Cairo, to use Morus nigra leaves extract for the green synthesis of silver nanoparticles (AgNPs) and to evaluate the antifungal potency and anti-aflatoxin production of the synthesized AgNPs
The results of isolation and identification of seed borne mycoflora revealed that 16 different fungal species belonging to 7 genera were obtained mainly: Fusarium verticillioides; F. oxysporum; Fusarium spp.; Aspergillus flavus; A. niger; A. terreus; A. parasiticus; A. candidus; A. tamari; A. sydowii; Penicillium carryophylum; Alternaria sp.; Mucor spp.; Gliocladium fimbriatum; Rhizopus stolonifer and Rhizopus sp
Summary
Seed-borne fungi have been found to affect the growth and productivity of crop plants including legumes and cereals (Weber et al, 2001), in addition some species can produce health-damaging mycotoxins. The mycotoxins contamination in animal feed and human food represent a serious health and economic problem worldwide. Mycotoxins are secondary metabolites of several species of fungi. They are unavoidable and their production is depending on the environmental factors either in the field or during storage (Stoev, 2013). Aflatoxin B1 (AFB1) is known to be the most significant in terms of animal and human health risk (CAST, 2003; Bryden, 2012). Synthetic fungicides have main disadvantages such as; the accumulation in the ecosystem and the development of pesticide resistance pathogens (Okigbo, 2004)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
