Green synthesis of Ceriumoxide Nanoparticles using Bacopa monnieri Leaf Extract - In vitro A ntioxidant Activity

Abstract

Objectives: Cerium oxide nanoparticles were synthesized by a rapid green method using an aqueous leaf extract of Bacopa monnieri to investigate its antioxidant activity. Methods: Cerium oxide nanoparticles (CeO2 NPs) were synthesized using Bacopa monnieri leaf extract as a stabilizing agent by solution combustion method. Characterization techniques like FTIR, PXRD,SEMEDAX, HR-TEM, UV-visible, and Raman spectroscopy confirmed the synthesized NPs. The antioxidant capacity was assessed by in vitro Hydroxyl radical scavenging assay. Findings: The biosynthesized CeO2 NPs possess tiny spherical shapes and sizes ranging from 5-50nm, and the average particle size is 27 nm as measured by HR-TEM. XRD and Raman confirm the nanoparticle’s polycrystalline nature and face-centered cubic fluorite structure with maximum UV-visible absorbance at l max 301.2nm. The biosynthesized CeO2 NPs exhibited better scavenging activity than Vitamin C. However, further in vitro and in vivo studies must be warranted to explore its mechanism and therapeutic potential. Novelty: To the best of our knowledge, this is the first report involving the green synthesis of cerium oxide nanoparticles using Bacopa monnieri leaf extract, which exhibited potent hydroxyl radical scavenging activity which can be a promising therapeutic candidate in oxidative stress-related disorders. Keywords: CeO2 NPs; Green synthesis; Bacopa monnieri; HR-TEM; Hydroxyl radical